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TIRF Fluorometer |
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TF1002-01 |
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Summary:
 Stand-alone TIRF biosensor instrument
Ultimate sensitivity and selectivity
Computer-controlled fluidics and filters
LED illuminator
User friendly software with TIRF
experiment templates and wizards
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| Overview: |
TF1002 is a total internal reflection fluorescence (TIRF) fluorometer equipped with a computer-controlled fluidic system. The sensitivity of the TIRF Fluorometer TF1002 is at the level of single molecule detection. TF1002 is a cost effective system with manual docking of TIRF slides and manual loading of bioanalyte solutions. The system is supplied with chemically modified silica TIRF slides and reagent kits for convenient immobilization of different biomolecules.
No other technique exists that can monitor fluorescence polarization, quenching, resonance energy transfer (FRET), recovery after photobleaching (FRAP), and fluorescence correlation spectroscopy (FCS) for analysis of biomolecular interactions in real-time and with sensitivity at the level of single-molecule detection. Electrochemical and temperature control are available as options. Chemically clean TIRF slides and chemically modified slides with reactive amine, aldehyde, epoxy groups, biotinylited and streptavidin coated TIRF slides are available as consumables. Chemically modified TIRF slides are supplied with reagent kits and protocols for surface immobilization of biomolecules such as proteins, lipids and nucleic acids.
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| Specifications: |
| Type of data |
Kinetics of biomolecular interactions, kon and koff rate constants, affinity constants, monitoring structural changes. |
| Type of measurement |
Real-time, non-destructive, measurement of fluorescence in TIRF mode by photon counting PMT. |
| Wavelength range |
280 - 700 nm. |
| Illuminators |
Internal illuminators: LED, laser diodes, or a halogen lamp. External illuminator: fiber optics Xenon lamp. |
| Photodetector |
Photon-counting PMT. |
| TIRF sensor chips |
Silica or glass 1" x 3" microscopy slides with chemically modified surfaces and reagent kits. |
| Bioanalyte type |
Solutions of biomolecules. Fluorescent reporter can be located either at the receptor molecule or at the ligand. |
| Liquids handling |
Computer controlled priming, sample injection and rinsing. Manual loading of bioanalyte and regeneration solutions. |
| Sample volume |
100-1000µl. |
| Sample recovery |
Computer-controlled. |
| Flow chamber volume |
10 µl. |
| Data representation |
Real time graph. |
| Size /Weight |
9"H x 13"W x 10"L (23cmH x 33cmW x 25cmL)/20lb |
| Clearance |
From back wall - 3" (8cm), from top - 5" (8cm). |
| Warranty and Support |
Off-site, included for one year. On-site and extended off-site warranty available for fee. |
| PC requirements |
Windows XP, 2Ghz Processor, 512MB RAM, 100Mb available disk space, CD-ROM, USB2 port. |
| Power requirement |
120 AC, 250 W. |
| Optional accessories |
Chemically modified TIRF and TIRF-EC slides, reagent kits for immobilization of receptor molecules. Electrochemical control of the TIRF-EC sensor surface. Temperature control. Xenon lamp illuminator. Autosampler. |
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